Recombinant Human IL-18 Protein

重组人IL-18蛋白 | 重组人 IL-18 蛋白

Iboctadekin | IFN gamma inducing factor | IFN-gamma-inducing factor | IGIF | IL 1 gamma | IL 18 | IL 1g | IL-1 gamma | IL-18 | IL1 gamma | IL18 | IL18 protein | IL18_HUMAN | IL1F4 | IL1g | IL1gamma | ILIF4 | Interferon gamma inducing factor | Interferon g

rp156177-50μg

50μg

无载体

≥95%(SDS-PAGE)

>95% (SDS-PAGE)

Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils. In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells. IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst. In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis. Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18. Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation. A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin.

1. Immobilized Recombinant Human IL-18 Bpa Protein (rp169678) at 2.0 μg/mL can bind Recombinant Human IL-18 Protein (rp156177) with the EC50 of 24.67 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL-18 (rp156177) at 2.0 μg/mL can bind GSK 1070806 (anti-IL-18) (Ab177855).

重组表达

20.5 kDa

N-His

17.8 kDa, under reducing condition; 17.8 kDa, under non-reducing condition

E. coli Accession #: Q14116 | E. coli

<1.0 EU/μg

人(Human)

37-193 aa

MGSSHHHHHHSSGLVPRGSHMYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

No

Yes