Recombinant Human IGFBP-5 Protein

重组人IGFBP-5蛋白 | 重组人胰岛素样生长因子结合蛋白5

IBP-5 | IGF-binding protein 5 | IGFBP5 | IGFBP-5 | insulin-like growth factor binding protein 5 | insulin-like growth factor-binding protein 5

rp167866-10μg

10μg

无动物源

≥90%(SDS-PAGE)

Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling. Post-translational: Cleaved by C1S in extracellular space.

1、Measured by its ability to inhibit the biological activity of IGF-I on MCF‑7 human breast cancer cells. The ED50 for this effect is 73.91 ng/mL in the presence of 2 ng/mL Recombinant Human IGF-1 Protein (rp147252). 2、Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-5 Protein (rp167866) at 1 μg/mL can bind Human IGF-1 Protein, the ED50 for this effect is 88.81 ng/mL.

重组表达

28-29 kDa

C-His

30 - 37 kDa, under reducing conditions; 30 - 37 kDa, under non-reducing conditions.

HEK293 Accession #: P24593 | HEK293

<0.1 EU/μg

人(Human)

21-272 aa

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVEHHHHHH

Yes

Yes