Recombinant Human Fas/TNFRSF6/CD95 Protein

重组人Fas/TNFRSF6/CD95蛋白 | 重组人凋亡相关因子蛋白 | 肿瘤坏死因子受体超家族成员6

Apo-1 antigen | Apo-1 | apoptosis antigen 1 | Apoptosis-mediating surface antigen FAS | APT1 | APT1FASTM | CD95 antigen | CD95 | CD95ALPS1A | Fas (TNF receptor superfamily | Fas AMA | Fas antigen | Fas | FAS1 | FASLG receptor | TNFRSF6 | TNFRSF6member 6 |

rp169551-10μg

10μg

无动物源

≥95%(SDS-PAGE)

CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD. Post-translational: (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Immobilized Recombinant Human Fas/TNFRSF6/CD95 Protein (rp169551) at 1.0 μg/mL can bind Fas Antibody (Ab102968) with the EC50 of 199.2 ng/mL.

重组表达

17.5 kDa.

C-His

22.7-39.5 kDa, under reducing conditions; 19.9-39.5 kDa, under non-reducing conditions.

HEK293 Accession #: P25445 | HEK293

<1.0 EU/μg

人(Human)

26-173 aa

QVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNHHHHHH

Yes

Yes