Recombinant Human CD28 Protein

重组人CD28蛋白 | 重组人T细胞表面糖蛋白CD28

CD28

rp169576-10μg

10μg

无动物源

≥95%(SDS-PAGE)

CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation. Post-translational: Phosphorylated by LCK (PubMed:7568038). Dephosphorylated by PTPN11 (PubMed:36114179). Tyrosine phosphorylated induced by CD40LG.

Immobilized Recombinant Human CD28 Protein (rp169576) at 1.0 μg/mL can bind Theralizumab (anti-CD28) (Ab190136) with the EC50 of 75.6 ng/mL.

重组表达

42.7 kDa

C-hFc & His

54.2-71.1 kDa, under reducing conditions; 109.6-148.9 kDa, under non-reducing conditions.

HEK293 Accession #: P10747 | HEK293

<1.0 EU/μg

人(Human)

1-152 aa

MLRLLLALNLFPSIQVTGNKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPENLYFQGPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH

Yes

Yes