Recombinant Human Pro-Collagen I alpha 1/COL1A1 Protein

重组人 Pro-Collagen I alpha 1/COL1A1蛋白 | 重组人I型胶原前体蛋白1 | 重组人类前胶原蛋白 I alpha 1/COL1A1 蛋白

COL1A1 | Alpha-1 type I collagen | COL-IA1 | Collagen 1 alpha 1 | Collagen 1 | collagen alpha 1 chain type I | collagen alpha-1(I) chain | Collagen I alpha 1 | collagen of skin, tendon and bone, alpha-1 chain | Collagen Type I Alpha 1 Chain | collagen, ty

rp169600-10μg

10μg

无动物源

≥90%(SDS-PAGE)

Protein Function： Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

Immobilized Recombinant Human COL1A1 Protein (rp169600) at 1.0 μg/mL can bind Collagen I Antibody (Ab097170) with the EC50 of 29.15 ng/mL.

重组表达

65.4 kDa

N-His

77.9 kDa under reducing conditions; above 250.0 kDa, under non-reducing conditions

HEK293 Accession #: P02452 | HEK293

<1.0 EU/μg

人(Human)

23-277 aa + 1094-1464 aa

HHHHHHQEEGQVEGQDEDIPPITCVQNGLRYHDRDVWKPEPCRICVCDNGKVLCDDVICDETKNCPGAEVPEGECCPVCPDGSESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQLSYGYDEKSTGGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGAPGKDGLNGLPGPIGPPGPRGRTGDAGPVGPPGPPGPPGPPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKDKRHVWFGESMTDGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPVCFL

Yes

Yes