Recombinant Human IL-16 Protein

重组人IL-16蛋白 | 重组人白细胞介素-16蛋白

FLJ16806 | FLJ42735 | FLJ44234 | HsT19289 | IL16 | IL-16 | interleukin 16 (lymphocyte chemoattractant factor) | LCF | LCFprIL-16 | lymphocyte chemoattractant factor | neuronal interleukin 16 | NIL16 | PRIL16 | prointerleukin 16 | pro-interleukin-16

rp169623-10μg

10μg

无载体

≥95%(SDS-PAGE)

Interleukin 16, also named lymphocyte chemoattractant factor (LCF), was originally identified as a CD8+ T-cell-derived chemoattractant for CD4+ cells. The biologically active form of IL-16 was originally proposed to be a homotetramer of 14 kDa chains containing 130 amino acid residue subunits. The complete pro-IL-16 cDNA was subsequently cloned and shown to encode a 631 amino acid residue hydrophilic protein that lacked a signal peptide. The original 130 amino acid residue polypeptide is now believed to have been derived from the C terminus of the precursor. IL-16 precursor protein has been detected in the lysates of various cells including mitogen stimulated PBMCs. The biologically active and secreted natural IL-16 is assumed to be a proteolytic cleavage product of pro-IL-16 generated by proteases present in or on activated CD8+ cells. A likely cleavage site was proposed to be at aspartate residue 510. This would yield a 121 amino acid residue protein, smaller than the 130 aa residue protein first described. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The gene for IL-16 precursor has been localized to chromosome 15. The biological activities ascribed to IL-16 are reported to be dependent on the cell surface expression of CD4, suggesting that IL-16 is a CD4 ligand. Besides its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro. Recombinant E. coli-derived IL-16 produced at R&D Systems is present mostly as a monomer, exhibits chemotactic activity for lymphocytes at high concentrations, lacks chemotactic activites for monocytes, and binds the extracellular domain of CD4 with low affinity. Post-translational: Synthesized as a chemo-attractant inactive precursor in hemopoietic tissues, and proteolytically cleaved by caspase-3 to yield IL-16.

Testing in progress

重组表达

14.2 kDa

C-His

17.3 kDa, under reducing conditions; 17.5 & 37.9 kDa, under non-reducing conditions.

E. coli Accession #: Q14005 | E. coli

<1.0 EU/μg

人(Human)

1203-1332 aa

MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDSHHHHHH

No

Yes