Recombinant Human BAFF/BLyS/TNFSF13B Protein

重组人BAFF/BLyS/TNFSF13B蛋白 | 重组人肿瘤坏死因子配体超家族成员13B蛋白 | 重组人B细胞活化因子蛋白 | 重组人B淋巴细胞刺激因子蛋白

ApoL related ligand TALL-1 | B lymphocyte stimulator | BAFF | BAFFB-cell activating factor | B-cell-activating factor | BLyS | BLYSB-lymphocyte stimulator | CD257 antigen | CD257 | Dendritic cell-derived TNF-like molecule | DTL | TALL1 | TALL-1delta BAFF

rp169674-10μg

10μg

无载体

≥90%(SDS-PAGE)

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL. A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134. Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation. Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function. BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes. Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R. All three receptors are primarily expressed by B cells. BAFF plays a critical role in the development and survival of B lineage cells. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders. In addition, BAFF co-stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells. BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages. Post-translational: The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated.

Measured in a cell proliferation assay using anti-IgM (115-006-020) stimulated mouse B cells. The ED50 for this effect is 0.78 ng/mL in the presence of 800 ng/mL anti-IgM. Immobilized Recombinant Human BAFF/BLyS/TNFSF13B Protein (rp169674) at 1.0 μg/mL can bind BAFF Antibody (Ab090522) with the EC50 of 346.4 ng/mL.

重组表达

17.1 kDa

No tag

16.1 kDa, under reducing conditions; 14.6 & 16.5 & 34.5 kDa, under non-reducing conditions.

E. coli Accession #: Q9Y275 | E. coli

<1.0 EU/μg

人(Human)

134-285 aa

GAVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

No

Yes