Recombinant Human HRV 3C Protein

重组人HRV 3C蛋白 | 重组人HRV 3C蛋白

HRV-3C Protease | 3C Protease

rp170396-1KU

1KU

无载体

≥95%(SDS-PAGE)

>95% (SDS-PAGE)

HRV 3C蛋白酶是人鼻病毒3C (HRV 3C)的重组半胱氨酸蛋白酶，在大肠杆菌中表达并纯化。HRV 3C蛋白酶在Gln和Gly残基之间以识别序列Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro切割蛋白质底物。该蛋白酶的高特异性和高亲和力标签(6xHis)使其成为去除重组蛋白纯化和检测标签的理想选择，并能灵活去除该蛋白酶。 来源： HRV 3C蛋白酶是在大肠杆菌中表达并纯化的一种人鼻病毒3C (HRV 3C)的重组半胱氨酸蛋白酶。 HRV 3C酶切His-GST-IL33蛋白，按照质量比（HRV 3C：目的蛋白）1：25和1：50酶切，4℃过夜酶切结果如下：完全酶切干净。 Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C (HRV 3C)expressed in and purified from Escherichia coli. HRV 3C Protease cleaves protein substrates with the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro between the Gln and Gly residues. The high specificity and affinity tags( 6xHis) of the protease make it an ideal choice for the removal of purification and detection tags on recombinant proteins and allows for flexibility in protease removal. Source： HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C (HRV 3C) expressed in and purified from Escherichia coli. HRV 3C enzyme digestion of His-GST-IL33 protein, according to the mass ratio (HRV 3C: target protein) 1:25 and 1:50 enzyme digestion, overnight at 4℃ enzyme digestion results are as follows: completely clean enzyme digestion

2.5 Units/μg Unit definition:One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 95% completion at 4°C in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, pH 7.0.

重组表达

22.3 kDa

N-His

24.0 kDa, reducing conditions; 24.0 kDa, non-reducing conditions

E. coli Accession #: P03303 | E. coli

<1.0 EU/μg

人(Human)

1538-1719 aa

MGSSHHHHHHSSGLVPRGSHMGPNTEFALSLLRKNIMTITTSKGEFTGLGIHDRVCVIPTHAQPGDDVLVNGQKIRVKDKYKLVDPENINLELTVLTLDRNEKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAGLINLSSTPTNRMIRYDYATKTGQCGGVLCATGKIFGIHVGGNGRQGFSAQLKKQYFVEKQ.

No

Yes