Recombinant Human FGF acidic/FGF1 Protein

重组人FGF acidic/FGF1蛋白 | 重组人 FGF 酸性/FGF1 蛋白

AFGF | alpha | alpha-ECGF | beta-ECGF | ECGF | ECGFB | ECGF-betaAcidic fibroblast growth factor | endothelial cell growth factor, beta | FGF acidic | FGF-1 | FGFABeta-endothelial cell growth factor | FGF-alpha | fibroblast growth factor 1 (acidic) | GLIO7

rp170413-10μg

10μg

无载体

≥95%(SDS-PAGE)

Protein Function: FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%‑33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1. It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes. Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals. The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of 10 µg/mL heparin sodium (H104201). The ED50 for this effect is typically 0.28 ng/mL.

重组表达

15.9 kDa

No tag

14.3 kDa, under reducing conditions; 14.2 kDa, under non-reducing conditions

E. coli Accession #: P05230 | E. coli

<1.0 EU/μg

人(Human)

16-155 aa

FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

No

Yes