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Product details

名称

Recombinant Human HGF Protein

别名

重组人HGF蛋白, 重组人肝细胞生长因子. | 重组人HGF蛋白

英文别名

货号

rp175908-50μg

包装规格

50μg

级别

无动物源

浓度

≥95%(SDS-PAGE)

生化机理

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It's a multidomain molecule with an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain lacking detectable protease activity. Human HGF is secreted as an inactive single chain propeptide and cleaved to form a bioactive disulfide-linked molecule with alpha and beta chains. Alternate splicing generates human HGF isoforms with variations in the Kringle domains. Human HGF shares high amino acid sequence identity with other mammals. HGF binds to heparan-sulfate proteoglycans and the receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in cancer development. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells and can alter epithelium morphology. In the thyroid, HGF influences thyrocyte behavior and inhibits iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium. Post-translational: The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.

生物活性

1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC50 for this effect is 97.73 ng/mL.

来源

重组表达

预测分子量

80.5 kDa

蛋白标签

C-His

SDS-PAGE

30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa, under reducing condition; 67.0-77.2 kDa, under non-reducing condition

表达系统

HEK293 Accession #: P14210 | HEK293

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

1-728 aa (D384N, D416N, L622S, H645R, V678I)

序列

MWVTKLLPALLLQHVLLHLLLLPIAIPYAEGQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCNMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWNKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGSINYDGLLRVAHLYIMGNEKCSQRHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQSHHHHHH

无动物源

Yes

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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