Recombinant Bovine CD14 Protein

重组人牛CD14蛋白

CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein

rp175913-10μg

10μg

无动物源

≥95%(SDS-PAGE)

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

重组表达

40.5 kDa

C-His

43.9-52.5 kDa, under reducing conditions; 41.9-49.3 kDa, under non-reducing conditions.

HEK293 Accession #: Q95122 | HEK293

<1.0 EU/μg

牛(Bovine)

1-373 aa

MVCVPYLLLLLLPSLLRVSADTTEPCELDDDDFRCVCNFTDPKPDWSSAVQCMVAVEVEISAGGRSLEQFLKGADTNPKQYADTIKALRVRRLKLGAAQVPAQLLVAVLRALGYSRLKELTLEDLEVTGPTPPTPLEAAGPALTTLSLRNVSWTTGGAWLGELQQWLKPGLRVLNIAQAHSLAFPCAGLSTFEALTTLDLSDNPSLGDSGLMAALCPNKFPALQYLALRNAGMETPSGVCAALAAARVQPQSLDLSHNSLRVTAPGATRCVWPSALRSLNLSFAGLEQVPKGLPPKLSVLDLSCNKLSREPRRDELPEVNDLTLDGNPFLDPGALQHQNDPMISGVVPACARSALTMGVSGALALLQGARGFAHHHHHH

Yes

Yes