Recombinant Human S100A8/S100A9 Heterodimer Protein

重组人S100A8/S100A9 Heterodimer蛋白 | 重组人S100A8/S100A9 异二聚体蛋白

60B8AG | CAGA | calgranulin A | Calprotectin L1L subunit | Calprotectin | CFAG | CGLA | CP-10 | Cystic fibrosis antigen | L1AG | Leukocyte L1 complex light chain | MA387 | MIF | Migration inhibitory factor-related protein 8 | MRP8 | NIF | p8 | protein S10

rp179786-10μg

10μg

无载体

≥90%(SDS-PAGE)

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins. They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid. The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively. S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers. The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria. The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation. S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes. Post-translational: Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex (PubMed:25417112). Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.

Testing in progress

重组表达

11.7 & 14.1 kDa

C-His

10.8 &13.2 kDa, under reducing conditions; 10.8 &13.2 kDa, under non-reducing conditions.

E. coli Accession #: P06702,P05109 | E. coli

<1.0 EU/μg

人(Human)

1-93 aa (S100A8) & 1-114 aa (S100A9)

MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKEHHHHHH MTCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTPHHHHHH

No

Yes