Recombinant Human CCL23/MPIF-1 Protein

重组人CCL23/MPIF-1蛋白 | 重组人骨髓抑制因子1蛋白

CCL23 | CCL23/MPIF-1 | CKb8 | Ckb-8 | Ckb-8-1 | CK-BETA-8 | hmrp-2a | MIP3 | MIP-3 | MPIF1 | MPIF-1 | SCYA23

rp179826-10μg

10μg

无载体

≥95%(SDS-PAGE)

Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors. Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than CCL23. Post-translational: The N-terminal is proteolytically cleaved by proteases associated with inflammatory responses. The processed forms, CCL23(19-99), CCL23(22-99), CCL23(27-99) and CCL23(30-99) exhibit increase in CCR1-mediated signaling and chemotaxis assays in vitro.

Determined by its ability to chemoattract human T cells population using a concentration range of 10.0-100.0 ng/mL.

重组表达

12.3 kDa

No tag

12.1 kDa, under reducing conditions; 12.3 & 17.1 kDa, under non-reducing conditions.

E. coli Accession #: P55773 | E. coli

<1.0 EU/μg

人(Human)

22-120 aa

GRVTKDAETEFMMSKLPLENPVLLDRFHATSADCCISYTPRSIPCSLLESYFETNSECSKPGVIFLTKKGRRFCANPSDKQVQVCMRMLKLDTRIKTRKN

No

Yes