Recombinant Human FGF-9 Protein

重组人FGF-9蛋白 | 重组人成纤维细胞生长因子9蛋白

FGF9 | FGF-9 | fibroblast growth factor 9 (glia-activating factor) | Fibroblast growth factor 9 | GAF | glia-activating factor | HBFG-9 | HBGF-9 | Heparin-binding growth factor 9 | MGC119914 | MGC119915 | SYNS3

rp181240-10μg

10μg

无载体

≥95%(SDS-PAGE)

Fibroblast growth factor 9 (FGF-9), also known as HBGF9 and GAF, is a member of the FGF family of secreted glycoproteins involved in mammalian skeleton morphogenesis and growth. The FGF family is characterized by a core heparin-binding FGF domain of approximately 120 amino acids (aa) that exhibits a beta-trefoil structure. Mature mouse FGF-9 shares 99% and 100% aa sequence identity with human and rat FGF-9, respectively. FGF-9, along with FGF-16 and -20, form an FGF subfamily that shares 65-71% aa sequence identity, binds FGFR3(IIIb), and is efficiently secreted despite having an uncleavable, bipartite signal sequence. In addition to FGFR3(IIIb), FGF-9 binding to the IIIc splice forms of FGFR-1, -2, and -3 has been reported. In the mouse embryo, the location and timing of FGF-9 expression affects the development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes. Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia and causes rhizomelia, or shortening of the proximal skeleton. Additionally, a mutation in mouse FGF-9 is responsible for Elbow knee synostosis (Eks), which causes joint fusions in the elbow and knee. In humans, FGF9 mutations that lower receptor binding can result in multiple synostoses syndrome (SYNS). Altered FGF-9 expression or function is reported in human colon, endometrial, and ovarian cancers, correlating with progression, invasiveness, and survival. Post-translational: Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step. N-glycosylated.

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.36 ng/mL.

重组表达

24.2 kDa

N-His

25.5 kDa, under reducing conditions; 23.9 & 25.8 kDa, under non-reducing conditions.

E. coli Accession #: P31371 | E. coli

<1.0 EU/μg

人(Human)

3-208 aa

MHHHHHHPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS

No

Yes