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Product details

名称

Recombinant Human VISTA/B7-H5 Protein

别名

重组人VISTA/B7-H5/PD-1H蛋白 | 重组人 VISTA/B7-H5 蛋白

英文别名

货号

rp181308-10μg

包装规格

10μg

级别

无动物源

浓度

≥95%(SDS-PAGE)

产品介绍

Protein Function: Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules. Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively. The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane. Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure. It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4. Gi24 is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is up-regulated in vivo on activated monocytes and dendritic cells. Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, Gi24 limits disease progression in the autoimmune disease model EAE. Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance.

生物活性

Immobilized Recombinant Human VISTA/B7-H5/PD-1H Protein (rp181308) at 1.0 μg/mL can bind Onvatilimab (anti-VISTA) (Ab170685) with the EC50 of 48.93 ng/mL.

来源

重组表达

预测分子量

22.2 kDa

蛋白标签

C-His

SDS-PAGE

33.0-46.3 kDa, under reducing conditions; 31.3-43.6 kDa, under non-reducing conditions

表达系统

HEK293 Accession #: Q9H7M9 | HEK293

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

1-194 aa

序列

MGVPTALEAGSWRWGSLLFALFLAASLGPVAAFKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAAHHHHHH

无动物源

Yes

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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