Recombinant Human IL-12/IL-23 p40 Protein

重组人IL-12 p40蛋白 | 重组人IL-23 p40蛋白 | 重组人 IL-12/IL-23 p40 蛋白

CLMF | CLMF p40 | CLMF2 | Cytotoxic lymphocyte maturation factor 40 kDa subunit | IL12 p40 | IL-12 p40 | IL-12 subunit p40 | IL12B | IL-12B | IL-12BNK cell stimulatory factor chain 2 | interleukin 12, p40 | interleukin 12B (natural killer cell stimulatory

rp181556-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Protein Function: Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Immobilized Recombinant Human IL-12/IL-23 p40 Protein (rp181556) at 1.0 μg/mL can bind Briakinumab (anti-IL-12) (Ab175480) with the EC50 of 17.82 ng/mL.

重组表达

36.4 kDa

C-His

40.0-44.3 kDa, under reducing conditions; 36.5-41.0 kDa, under non-reducing conditions

HEK293 Accession #: P29460 | HEK293

<1.0 EU/μg

人(Human)

1-328 aa

MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCSEDLYFQSHHHHHH

Yes

Yes