Recombinant Human CDCP1 Protein

重组人CDCP1蛋白 | 重组人CUB结构域包含蛋白1

CD318 antigen | CD318 | CDCP1 | CUB domain containing protein 1 | CUB domain-containing protein 1 | Membrane glycoprotein gp140 | SIMA135 | SIMA135TRASK | Subtractive immunization M plus HEp3-associated 135 kDa protein | Transmembrane and associated with

rp181598-10μg

10μg

无动物源

≥90%(SDS-PAGE)

CDCP1 (CUB-domain containing protein 1), also known as Trask or CD318, is a type I transmembrane receptor whose specific role remains unclear. Human CDCP1 is synthesized with a large extracellular domain (ECD) containing three CUB domains, a transmembrane domain and a cytoplasmic domain with 5 tyrosine phosphorylation sites. Full-length CDCP1 (Isoform 1) is ~135 kDa and can be cleaved into a ~70 kDa membrane-bound and a ~65 kDa circulating form. A secreted only form of human CDCP1, called Isoform 2, can also be expressed consisting of amino acids (aa) 30-343 of the ECD. The mature ECD of human Isoform 1 shares 84% aa sequence identity to mouse and rat CDCP1 Isoform 1. CDCP1 was originally identified from proteins involved in metastasis and has been found on tumor, stem cells, keratinocytes and colonic epithelial cells. Tyrosine phosphorylation of the intracellular domain of CDCP1 results in downstream signaling through Src-family kinases (SFKs), Akt, and PKCδ. CDCP1 appears to be important for cell-cell and cell-substratum adhesion and the phosphorylation state of CDCP1 regulates this effect. CDCP1 is associated with a poor prognosis in epithelial tumors, such as lung, pancreatic, colorectal, renal, and ovarian carcinomas and is a novel marker of the most aggressive human triple-negative breast cancers. Several different extracellular forms of CDCP1 have been shown to exhibit disease-specific expression in prostate cancer. Post-translational: Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5. N-glycosylated. A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.

Testing in progress

重组表达

72.5 kDa

C-His

92.3-115.3 kDa, under reducing conditions; 86.7-107.0 kDa, under non-reducing conditions.

HEK293 Accession #: Q9H5V8 | HEK293

<1.0 EU/μg

人(Human)

1-666 aa

MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDLHHHHHH

Yes

Yes