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Product details

名称

Recombinant Human BID Protein

别名

重组人BID蛋白

英文别名

货号

rp183602-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

生化机理

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is a pro‑apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane. Cleavage of BID by caspase-8 generates a new N-terminus that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria. BID may then interact with another pro‑apoptotic Bcl-2 family member Bak. Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9-13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl‑xL. Post-translational: BH3-interacting domain death agonist: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622). BH3-interacting domain death agonist p15: Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

生物活性

Immobilized Recombinant Human BID Protein (rp183602) at 2.0 μg/mL can bind Recombinant Human Bcl-XL Protein (rp209934) with the EC50 of 261.4 ng/mL.

来源

重组表达

预测分子量

21.9 kDa

蛋白标签

No tag

SDS-PAGE

19.3 kDa, under reducing conditions; 19.3 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: P55957 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

2-195 aa

序列

DCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLARNGMD

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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