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Product details

名称

Recombinant Human B3GAT3 Protein

别名

重组人B3GAT3蛋白 | 重组人β-1,3-葡萄糖醛酸基转移酶3蛋白

英文别名

货号

rp183658-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

生化机理

B3GAT3 is an essential enzyme involved in the synthesis of the linkage region of heparan sulfate and chondroitin sulfate proteoglycans. It transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcA) to the trisaccharide Gal beta 1-3Gal beta 1-4Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. B3GAT3 knockout mice are embryonic lethal before the 8-cell stage due to failed cytokinesis, suggesting critical roles for chondroitin sulfate and heparan sulfate in embryonic cell division. Unlike the broad substrate specificity exhibited by B3GAT1, B3GAT3 shows strict specificity for Gal beta 1-3Gal beta 1. The crystal structure shows the enzyme is an alpha / beta protein with two subdomains that constitute the donor and acceptor binding sites with the active site lying in the cleft between the two subdomains. Like most known glycosyltransferases, B3GAT3 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single-pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. In the current assay, hydrolase activity against UDP-GlcA was measured using a phosphatase-coupled method. Post-translational: N-glycosylated.

生物活性

Measured by its ability to hydrolyze UDP-GlcA. The specific activity is >1 pmol/min/μg, as measured under the described conditions.

来源

重组表达

预测分子量

30.2 kDa

蛋白标签

N-His

SDS-PAGE

31.2 & 63.4 kDa, under reducing conditions; 31.2 & 63.4 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: O94766 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

72-335 aa

序列

MHHHHHHEALPTIYVVTPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAEGPTPLVSGLLAASGLLFTHLVVLTPKAQRLREGEPGWVHPRGVEQRNKALDWLRGRGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFEEMRWTRGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPSRPFPVDMAGFAVALPLLLDKPNAQFDSTAPRGHLESSLLSHLVDPKDLEPRAANCTRVLVWHTRTEKPKMKQEEQLQRQGRGSDPAIEV

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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