Recombinant Human PKLR Protein

重组人PKLR蛋白 | 重组人丙酮酸激酶PKLR蛋白

EC 2.7.1.40 | PK1 | PK1PKRL | PKL | PKLR | PKR | Pyruvate Kinase 1 | pyruvate kinase isozyme R/L | pyruvate kinase isozymes R/L | pyruvate kinase type L | pyruvate kinase | Red cell/liver pyruvate kinase | RPK | R-type/L-type pyruvate kinase | liver and b

rp183692-10μg

10μg

无载体

≥95%(SDS-PAGE)

Pyruvate kinases (PK) are glycolytic enzymes that catalyze the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP, the final step in the glycolysis pathway. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1, and M2. L type is the major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart, and brain, and M2 is found in early fetal tissues. R and L types are encoded in the same gene and caused by different splicing. The 33 amino acids from the N-terminus in the R type are replaced with Met and Glu in the L type. PKLR is allosterically activated by fructose 1,6-bisphosphate. Mutation of PKLR is a frequent cause of hereditary non-spherocytic hemolytic anemia.

重组表达

57.9 kDa

N-His

56.9 kDa, under reducing conditions; 56.9 kDa, under non-reducing conditions.

E. coli Accession #: P30613 | E. coli

<1.0 EU/μg

人(Human)

47-574 aa

MHHHHHHLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS

No

Yes