Recombinant Human PTP1B Protein

重组人PTP1B蛋白 | 重组人非受体型酪氨酸蛋白磷酸酶1蛋白

EC 3.1.3.48 | protein tyrosine phosphatase 1B | protein tyrosine phosphatase, non-receptor type 1 | Protein-tyrosine phosphatase 1B | PTP1B | PTP-1B | PTP1Bprotein tyrosine phosphatase, placental | PTPN1 | tyrosine-protein phosphatase non-receptor type 1

rp183698-10μg

10μg

无载体

≥90%(SDS-PAGE)

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This ubiquitously expressed enzyme is anchored in the endoplasmic reticulum by its C-terminal end and has its catalytic regions exposed to the cytosol. The recombinant protein lacks the C-terminal 114 amino acids but is fully active. PTP1B will dephosphorylate a wide variety of phosphoproteins, such as receptors for the growth factors insulin and epidermal growth factor (EGF), c-Src and beta -catenin. Of particular interest is the observation that PTP1B knock-out mice are resistant to high-caloric intake-induced obesity and have exaggerated insulin responses, suggesting that PTP1B may play an important role in regulating growth factor responsiveness. Post-translational: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

Immobilized Recombinant Human PTP1B Protein (rp183698) at 1.0 μg/mL can bind PTP1B Mouse mAb (Ab123873) with the EC50 of 14.2 ng/mL.

重组表达

38.1 kDa

N-His

35.3 kDa, under reducing conditions; 35.3 kDa, under non-reducing conditions.

E. coli Accession #: P18031 | E. coli

<1.0 EU/μg

人(Human)

2-321 aa

MHHHHHHEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHN

No

Yes