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Product details

名称

Recombinant Human UBE2I/UBC9 Protein

别名

重组人UBE2I/UBC9蛋白 | 重组人相扑结合酶UBC9蛋白

英文别名

货号

rp183712-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

生化机理

Ubiquitin-conjugating Enzyme E2I (UBE2I), also known as Ubiquitin-conjugating Enzyme 9 (Ubc9), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. Human UBE2I/Ubc9 shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2I/Ubc9 catalyzes the addition of the Ubiquitin-like protein SUMO to target proteins. SUMO is transferred from a Ubiquitin-like activating (E1) enzyme heterodimer consisting of SAE1 and UBA2 to Cys93 of UBE2I/Ubc9. In contrast to most Ubiquitin-conjugating (E2) enzymes that function in a complex with Ubiquitin ligases (E3s), UBE2I/Ubc9 can interact directly with protein substrates and may also play a role in substrate recognition. UBE2I/Ubc9 mediates the SUMOylation of a variety of proteins including RanGAP1, HDAC4, and PML. Post-translational modifications of UBE2I/Ubc9, such as auto-SUMOylation at Lys14 or Ser71 phosphorylation by CDC2/CDK1, alter UBE2I/Ubc9 catalytic activity and target protein recognition. UBE2I/Ubc9-dependent SUMOylation reduces the levels of the stem cell marker Nanog, implicating it in embryonic stem cell pluripotency maintenance. SUMOylation also regulates the protein levels of tumor suppressors and oncogenes, and UBE2I/Ubc9 dysregulation is thought to contribute to the pathogenesis of human cancers. Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed:26620705). Post-translational: Phosphorylation at Ser-71 significantly enhances SUMOylation activity.

生物活性

Immobilized Recombinant Human UBE2I/UBC9 Protein (rp183712) at 1.0 μg/mL can bind Recombinant UBE2I Antibody (Ab133246) with the EC50 of 4.2 ng/mL.

来源

重组表达

预测分子量

17.9 kDa

蛋白标签

No tag

SDS-PAGE

15.7 kDa, under reducing conditions; 15.7 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: P63279 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

2-158 aa

序列

SGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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