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Product details

名称

Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein

别名

重组人/小鼠/大鼠UBE2M/Ubc12蛋白 | 重组人/小鼠/大鼠参与 NEDD8 偶联的 Ubc12 酶蛋白

英文别名

货号

rp183713-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

生化机理

Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins. Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme. The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression. UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family. Post-translational: The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.

生物活性

Immobilized Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) at 1.0 μg/mL can bind UBE2M/UBC12 Mouse mAb (Ab133254) with the EC50 of 25.4 ng/mL.

来源

重组表达

预测分子量

20.8 kDa

蛋白标签

No tag

SDS-PAGE

19.2 kDa, under reducing conditions; 19.2 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: P61081 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

2-183 aa

序列

IKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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