Recombinant Human LILRB3/CD85a/ILT5 Protein

重组人LILRB3/CD85a/ILT5蛋白 | 重组人白细胞免疫球蛋白样受体 B3

CD85 antigen-like family member A | CD85a antigen | CD85a | HL9Immunoglobulin-like transcript 5 | ILT5 | ILT-5 | ILT5CD85a | Leukocyte immunoglobulin-like receptor 3 | leukocyte immunoglobulin-like receptor subfamily B member 3 | leukocyte immunoglobulin-

rp184064-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. Subfamily A members are activating receptors that lack ITIMs and signal through association with FcR gamma. Mature LILRB3 is a highly polymorphic 85-95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs. Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein, which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors. Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells. On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation. LILRB3 can also bind to ligands exposed on necrotic tumor cells. Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs. R&D Systems in-house testing indicates that LILRB3 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families. In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration. Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons. Post-translational: Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-595 and Tyr-625 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2.

Immobilized Recombinant Human Angiopoietin-like 7/ANGPTL7 Protein (rp142833) at 5.0 μg/mL can bind Recombinant Human LILRB3/CD85a/ILT5 Protein (rp184064) with the EC50 of 444.7 ng/mL.

重组表达

46.9 kDa

C-His

54.2-71.3kDa, under reducing conditions; 54.2-71.3 kDa, under non-reducing conditions.

HEK293 Accession #: AAB68668 | HEK293

<1.0 EU/μg

人(Human)

1-443 aa

MTPALTALLCLGLSLGPRTRVQAGPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRLDKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGFYNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQALFPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQSLTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSPSHGGQYRCYGAHNLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSGHSGGSSLPPTGPPSTPGLGRYLEHHHHHH

Yes

Yes