Recombinant Human Semaphorin 7A Protein

重组人Semaphorin 7A蛋白 | 重组人信号素7A蛋白

CD108 antigen | CD108 | CD108MGC126696 | CDw108 | H-SEMA-K1 | H-Sema-L | JMH blood group antigen | JMH | John-Milton-Hargen human blood group Ag | MGC126692 | sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood grou

rp184082-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules. On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen. Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen, and thymus. Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86%, and 90% aa identity with mouse, rat, canine, bovine, and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif. Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1. Sema7A signaling through the two receptors may cause opposing effects. Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages, and microglia. T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation. However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses. In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta-induced lung fibrosis. Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract.

Immobilized Recombinant Human Semaphorin 7A Protein (rp184082) at 2.0 μg/mL can bind Semaphorin 7a Antibody (Ab127023) with the EC50 of 5.2 ng/mL.

重组表达

69.3 kDa

C-His

73.0 kDa, under reducing conditions; 72.1 & 128.0 kDa, under non-reducing conditions.

HEK293 Accession #: O75326 | HEK293

<1.0 EU/μg

人(Human)

1-648 aa

MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALAHHHHHH

Yes

Yes