Recombinant Human PDK1 Protein

重组人PDK1蛋白 | 重组人3-磷酸肌醇依赖性蛋白激酶1蛋白

3-phosphoinositide-dependent protein kinase 1 | 3-phosphoinositide-dependent protein kinase 2 pseudogene | PDK1 | PDK-1 | PDPK1 | PDPK2 | PDPK2P | PkB kinase like gene 1 | PKB kinase

rp184107-10μg

10μg

无载体

≥95%(SDS-PAGE)

PDK-1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns P3 or PtdIns P2. PDK‑1 then activates protein kinase B (PKB) that, in turn, inactivates glycogen synthase kinase-3 (GSK-3). The phosphorylation of other proteins by PKB and GSK-3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK‑1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns P3 and/or PtdIns P2. Human PDK‑1 is a 556 amino acid residue monomeric enzyme comprised of a catalytic domain that is most similar to the PKA, PKB, and PKC subfamily of protein kinases. Post-translational: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

Testing in progress

重组表达

18.3 kDa

N-His

16.4 kDa, under reducing conditions; 16.6 & 31.8 kDa, under non-reducing conditions.

E. coli Accession #: O15530 | E. coli

<1.0 EU/μg

人(Human)

411-556 aa

MHHHHHHNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ​

Yes

Yes