Recombinant Human Biliverdin Reductase B/BLVRB Protein

重组人Biliverdin Reductase B/BLVRB蛋白 | 重组人黄素还原酶(NADPH)蛋白

biliverdin reductase B (flavin reductase (NADPH)) | Biliverdin Reductase B | Biliverdin-IX beta-reductase | BLVRB | BVRB | BVR-B | EC 1.3.1.24 | EC 1.5.1.30 | flavin reductase (NADPH) | flavin reductase | FLR | FLRBVRB | FR | GHBP | Green heme-binding pro

rp184878-10μg

10μg

无载体

≥95%(SDS-PAGE)

Clearance of heme in mammals is a two-step process starting with the conversion of heme to biliverdin by heme oxygenase, followed by the reduction of biliverdin to bilirubin by biliverdin reductase. Biliverdin IX b reductase (BLVRB) converts the beta isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore, BLVRB is especially important for fetal heme metabolism and clearance. BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but it is present in other tissues. The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD, FMN, and met-hemoglobin. BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha isomer but could also use the beta isomer as a substrate, BLVRB is specific for the beta isomer.

Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor. The specific activity is >225.0 pmol/min/μg, as measured under the described conditions.

重组表达

22.9 kDa

N-His

24.8 kDa, under reducing conditions; 24.8 kDa, under non-reducing conditions.

E. coli Accession #: P30043 | E. coli

<1.0 EU/μg

人(Human)

2-206 aa

MHHHHHHAVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ

No

Yes