Recombinant Human Annexin A1/ANXA1 Protein

重组人Annexin A1/ANXA1蛋白 | 重组人膜联蛋白A1

Annexin A1 | annexin I (lipocortin I) | Annexin I | Annexin-1 | ANX1 | ANXA1 | Calpactin II | calpactin-2 | chromobindin-9 | Lipocortin I | LPC1 | LPC1annexin-1 | p35 | Phospholipase A2 inhibitory protein

rp184917-10μg

10μg

无载体

≥95%(SDS-PAGE)

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane‑associated proteins. They are characterized by multiple repeats of 60‑70 amino acids (aa) which assemble into a curved disc‑like domain that binds to membrane phospholipids in a calcium‑dependent manner. Human Annexin A1 (ANXA1), also known as Lipocortin 1, Calpactin II, Lipomodulin, and Chromobindin 9, contains four annexin repeats and shares approximately 88% aa sequence identity with mouse and rat Annexin A1. Annexin A1 is found in the cytoplasm, on the cell surface, and in the extracellular fluid. The full length 37 kDa protein is susceptible to intracellular and extracellular proteolytic cleavages which generate various N‑terminal peptides and a truncated C‑terminal portion. In addition to its membrane activities, Annexin A1 is a major effector of glucocorticoid‑induced immune suppression and growth arrest. It associates with S100A11, and this complex inhibits Phospholipase A2 and the production of inflammatory eicosanoids. Annexin A1 is released by apoptotic cells and also during neutrophil adhesion to vascular endothelial cells. It binds the FPRL1/ALX receptor on neutrophils, while the cleaved N‑terminal aa 2‑26 peptide additionally binds FPR. These interactions inhibit leukocyte activation and extravasation to sites of inflammation. In contrast, the 33 kDa molecule (generated by the removal of aa 2‑26) promotes activation of vascular endothelial cells and neutrophil transendothelial migration. Annexin A1 phosphorylation at multiple locations is important for the regulation of its cleavage, membrane interactions, and ability to suppress ACTH production in the pituitary. Annexin A1 additionally regulates adaptive immune responses by enhancing T cell activation and promoting a Th1 bias. It can exert both positive and negative effects on epithelial‑mesenchymal transition and tumor metastasis. Post-translational: Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment.

Immobilized Recombinant Human Annexin A1/ANXA1 Protein (rp184917) at 1.0 μg/mL can bind Annexin A1/ANXA1 Mouse mAb (Ab088935) with the EC50 of 520.9 ng/mL.

重组表达

39.5 kDa

C-His

37.8 kDa, under reducing conditions; 37.2 kDa, under non-reducing conditions.

E. coli Accession #: P04083 | E. coli

<1.0 EU/μg

人(Human)

1-346 aa

MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGNHHHHHH

No

Yes