Recombinant Human Epimorphin/Syntaxin 2 Protein

重组人Epimorphin/Syntaxin 2蛋白 | 重组人上皮成形素蛋白 | 重组突触融合2蛋白

EPIMepimorphin | Epimorphin | EPM | STX2 | STX2AMGC51014 | STX2Bsyntaxin-2 | STX2CEpimorphin | Syntaxin 2

rp184938-10μg

10μg

无载体

≥95%(SDS-PAGE)

Epimorphin (EPIM), also known as syntaxin 2 (STX2), is a type IV transmembrane protein that is a member of the syntaxin family of tSNARE (targetSoluble NSF Attachment REceptor) membrane fusion proteins. Intracellular Epimorphin functions as a vesicle fusion protein, but extracellular forms that are active in morphogenesis are also found. Human Epimorphin cDNA encodes 289 amino acids (aa) including a coiledcoil domain (aa 35 101), a potential cellrecognition sequence (aa 105 123), a sequence important for membrane transduction (aa 141 150), a tSNARE domain (aa 191 252), and a Cterminal transmembrane domain (aa 265 288). Within aa 1 188, human Epimorphin shares 89%, 88%, 94% and 95% aa sequence homology with mouse, rat, bovine and porcine Epimorphin, respectively. Potential isoforms of 287 and 277 aa show Cterminal substitutions that do and do not contain hydrophobic sequences, respectively. Epimorphin has no signal sequence, but cell stress or Ca2+ influx induces plasma membrane crossing, with the assistance of annexin A2 and synapatotagmin1. A soluble, extracellular 30 kDa form of Epimorphin is produced from the membrane–associated 34 kDa form by cleavage between E245 and H246. Intracellular 70 kDa and 150 kDa complexes are presumed to be dimers and tetramers, respectively. Epimorphin produced by mesenchymal cells influences morphogenesis of epithelia in the breast, kidney, intestine, lung, pancreas, liver, skin and intestines. For cells such as lung, mammary or pancreatic epithelia, soluble Epimorphin promotes tubulogenesis or hollow sphere formation in vitro, while epithelia plated on Epimorphin or Epimorphinproducing cells exhibit alpha v integrindependent adhesion and branching morphogenesis. Mice genetically lacking Epimorphin are sterile due to abnormal spermatogenesis and testicular development. Both endogenous and exogenous Epimorphin are shown to protect cells from oxidative stress.

Testing in progress

重组表达

21.6 kDa

No tag

22.4 kDa, under reducing conditions; 22.9 & 45.2 kDa, under non-reducing conditions.

E. coli Accession #: P32856 | E. coli

<1.0 EU/μg

人(Human)

2-188 aa

RDRLPDLTACRKNDDGDTVVVVEKDHFMDDFFHQVEEIRNSIDKITQYVEEVKKNHSIILSAPNPEGKIKEELEDLNKEIKKTANKIRAKLKAIEQSFDQDESGNRTSVDLRIRRTQHSVLSRKFVEAMAEYNEAQTLFRERSKGRIQRQLEITGRTTTDDELEEMLESGKPSIFTSDIISDSQITR

No

Yes