Recombinant Human Thymidine Kinase 1 Protein

重组人Thymidine Kinase 1蛋白 | 重组人胸苷激酶1蛋白 | 重组人胸苷激酶2蛋白

EC 2.7.1.21 | thymidine kinase 1 soluble isoform | Thymidine Kinase 1 | thymidine kinase 1, soluble | thymidine kinase, cytosolic | thymidine kinase-1 | TK1 | TK2

rp184947-10μg

10μg

无载体

≥90%(SDS-PAGE)

Thymidine kinases are 2'-deoxythymidine kinases that phosphorylate deoxythymidine and generate deoxythymidine 5’-phosphate. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Two forms of thymidine kinase are present in mammalian cells, TK1 and TK2. TK1 is cell cycle-dependent and is present in the cytoplasm only in anticipation of cell division; whereas TK2 is located in mitochondria and is cell cycle-independent. TK1 is synthesized by the cell during the S phase of cell division and is degraded after cell division is completed. TK1 normally occurs in tissue as a dimer and can be converted to more active tetrameric form by ATP binding. TK1 is feedback inhibited by thymidine triphosphate, the product of the further phosphorylation of thymidine. Because tumor cells replicate much more frequently than normal cells and requires faster DNA synthesis and higher TK1 activity, TK1 is a cancer maker especially for hematologic malignancies. In clinical chemistry TK1 is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease. In addition, thymidine kinase is required for the action of many antiviral drugs. The enzymatic activity of recombinant human TK1 is measured using a phosphatase-coupled method. Post-translational: Phosphorylated on Ser-13 in mitosis. Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level (PubMed:14697231, PubMed:9575153). Polyubiquitinated. Postmitosis, ubiquitination leads to proteasomal degradation. The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.

Immobilized Recombinant Human Thymidine Kinase 1 Protein (rp184947) at 2.0 μg/mL can bind Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) with the EC50 of 149.0 ng/mL.

重组表达

26.3 kDa

C-His

26.5 & 50.6 & 74.1 kDa, under reducing conditions; 24.2 & 42.1 & 72.9 kDa, under non-reducing conditions.

E. coli Accession #: P04183 | E. coli

<1.0 EU/μg

人(Human)

1-234 aa

MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPANHHHHHH

No

Yes