Recombinant Human SHP-2 Protein

重组人SHP-2蛋白

BPTP3 | CFC | JMML | METCDS | MGC14433 | NS1 | OTTHUMP00000166107 | OTTHUMP00000166108 | Protein tyrosine phosphatase 2 | Protein tyrosine phosphatase 2C | Protein tyrosine phosphatase non receptor type 11 | Protein-tyrosine phosphatase 1D | Protein-tyros

rp184964-10μg

10μg

无载体

≥95%(SDS-PAGE)

Src-Homology domain-2 containing protein tyrosine Phosphatase 2 (SHP-2), also called protein tyrosine phosphatase, non-receptor type 11 (PTPN11), PTP1D, PTP2C, and SYP, is an enzyme that dephosphorylates tyrosine residues in proteins. The protein contains two Src homology 2 (SH2) domains, which both regulate the activity of the enzyme and allow it to selectively bind to SH2 sites on proteins such as Dok1, IRS1, and the insulin receptor. SHP-2 plays a unique stimulatory role in cell signaling. Cells lacking SHP-2 have poor mobility because the hyper-phosphorylation of FAK and other proteins in the focal adhesion complex prevents turnover of cellular attachment points. Without SHP-2, sustained ERK stimulation does not take place. The Y992 phosphorylation site of EGFR is a particularly good substrate for SHP-2 and a phosphopeptide containing this sequence can be used to measure the activity of the enzyme (Catalog # ES006) by detecting release of phosphate (Catalog # DY996). Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Post-translational: Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

重组表达

68.8 kDa

N-His

62.2 kDa, under reducing conditions; 62.2 kDa, under non-reducing conditions.

E. coli Accession #: Q06124 | E. coli

<1.0 EU/μg

人(Human)

2-593 aa

MHHHHHHTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR

No

Yes