Recombinant Human NCAM-1/CD56 Protein

重组人CD56蛋白 | 重组人NCAM-1蛋白 | 重组人 NCAM-1/CD56 蛋白

CD56/NCAM-1 | CD56 antigen | CD56 | MSK39 | NCAM1 | N-CAM-1 | NCAM-1 | NCAMantigen recognized by monoclonal 5.1H11 | neural cell adhesion molecule 1 | neural cell adhesion molecule | NCAM

rp185696-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans, the extracellular matrix protein agrin, and several chondroitin sulfate proteoglycans that include neurocan and phosphocan. There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM‑140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM‑140 and NCAM-180 are type I transmembrane glycoproteins. Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180. NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment. The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features. NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity.

Measured by the ability of the immobilized protein to support the adhesion of SK-N-SH human neuroblastoma cells. The ED50 for this effect is 1.0 - 10.0 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human NCAM-1/CD56 Protein (rp175917) at 1.0 μg/mL can bind Lorvotuzumab (anti-CD56) with the EC50 of 28.65 ng/mL.

重组表达

65.5 kDa

C-His

92.1-121.6 kDa, reducing conditions; 77.5-110.5 kDa, non-reducing condition

HEK293 Accession #: P13591-3 | HEK293

<1.0 EU/μg

人(Human)

1-603 aa

MLQTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVHSPPPHHHHHH

Yes

Yes