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Product details
名称
Recombinant E. coli Glycerol kinase Protein
别名
重组大肠杆菌Glycerol kinase蛋白 | 重组大肠杆菌甘油激酶蛋白
英文别名
ATP glycerol 3 phosphotransferase | ATP: 3 phosphotransferase | ATP: 3-phosphotransferase | D930012N15Rik | GK | GK1 | GKD | GLPK_HUMAN | Glycerokinase | Glycerol kinase | OTTHUMP00000023108 | OTTHUMP00000023109 | OTTHUMP00000215321
货号
rp186498-10μg
包装规格
10μg
级别
无载体
浓度
≥90%(SDS-PAGE)
生化机理
来自大肠杆菌的甘油激酶(glpK)催化甘油的 ATP 依赖性磷酸化,生成 sn-甘油-3-磷酸酯(G3P),这是利用甘油的第一步,也是限制速率的一步。在甘油存在的情况下,glpK 通过与膜结合的甘油促进剂相互作用而受到刺激。在葡萄糖存在的情况下,glpK 的活性受到糖酵解途径中的 1,6-二磷酸果糖(FBP)的异构抑制。在生理条件下,该酶处于活性二聚体和非活性四聚体之间的平衡状态。FBP 与非活性形式结合并使其稳定,从而将甘油代谢途径的使用转移到糖酵解途径。GlpK 是 ATP 酶超家族的成员,该超家族包括肌动蛋白、己糖激酶和热休克蛋白 hsc70。虽然这些蛋白在氨基酸序列和功能上不尽相同,但它们具有相似的三级折叠,而且可能具有相同的催化机制。使用磷酸酶偶联激酶测定法测量了酶的活性。
生物活性
Testing in progress
来源
重组表达
预测分子量
57.0 kDa
蛋白标签
C-His
SDS-PAGE
52.3 kDa, under reducing conditions; 52.3 kDa, under non-reducing conditions.
表达系统
E. coli Accession #: P0A6F3 | E. coli
内毒素水平
<1.0 EU/μg
种属
大肠杆菌(E.coli)
氨基酸
1-502 aa
序列
MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDEHHHHHH
无动物源
No
无载体
Yes
Safety
「No Dangerous Attributes」
Related
「No upstream and downstream information yet」
Technical Documents
「No technical documents」
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「No related articles yet」
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Research chemical ≥90%

Research chemical ≥90%; supplied for laboratory research, analysis, inspection, and scientific procurement use; specifications: ≥90%.

item number:rp186498-10μg
Product model:10μg
level: ≥90%
Lead Time:30days
sold 807 Items
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