Recombinant Human HIF-2 alpha Protein

重组人HIF-2 alpha蛋白 | 重组人内皮PAS域蛋白1蛋白 | 重组人低氧诱导因子2α蛋白

Endothelial PAS domain-containing protein 1 | EPAS-1 | Basic-helix-loop-helix-PAS protein MOP2 | Class E basic helix-loop-helix protein 73 (bHLHe73) | HIF-1-alpha-like factor (HLF) | Hypoxia-inducible factor 2-alpha (HIF-2-alpha | HIF2-alpha) | Member of

rp186522-10μg

10μg

无载体

≥90%(SDS-PAGE)

Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity). Post-translational: In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

重组表达

36.5 kDa

N-His

36.7 & 77.9 kDa, under reducing conditions; 35.6 & 68.4-83.2 kDa, under non-reducing conditions.

E. coli Accession #: Q99814 | E. coli

<1.0 EU/μg

人(Human)

543-870 aa

MHHHHHHSPICPEERLLAENPQSTPQHCFSAMTNIFQPLAPVAPHSPFLLDKFQQQLESKKTEPEHRPMSSIFFDAGSKASLPPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWAVGDQRTEFLGAAPLGPPVSPPHVSTFKTRSAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRMKNLRGGSCPLMPDKPLSANVPNDKFTQNPMRGLGHPLRHLPLPQPPSAISPGENSKSRFPPQCYATQYQDYSLSSAHKVSGMASRLLGPSFESYLLPELTRYDCEVNVPVLGSSTLLQGGDLLRALDQAT

No

Yes