Recombinant Canine IL-2 (C147S) Protein

重组狗IL-2蛋白 | 重组狗白细胞介素2蛋白

Aldesleukin | IL2 | IL-2 | IL-2lymphokine | interleukin 2 | interleukin-2 | involved in regulation of T-cell clonal expansion | Proleukin | T cell growth factor | T-cell growth factor | TCGF

rp186528-10μg

10μg

无载体

≥90%(SDS-PAGE)

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes. The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits. IL-2 binds the alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the beta and gamma subunits, which is also capable of transducing IL-2 signals. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9. At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively.

Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is 18.45 ng/mL.

重组表达

15.4 kDa

13.3 kDa, under reducing conditions; 11.9 & 26.3 kDa, under non-reducing conditions.

E. coli Accession #: Q29416 | E. coli

<0.1 EU/μg

狗(Canine)

21-155 aa (C147S)

APITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT

No

Yes