Recombinant Human GM-CSF Protein

重组人GM-CSF蛋白 | 重组人粒细胞-巨噬细胞集落刺激因子

colony stimulating factor 2 (granulocyte-macrophage) | Colony-stimulating factor | CSF | CSF2 | CSF-2 | GMCSF | GM-CSF | GMCSFgranulocyte-macrophage colony-stimulating factor | granulocyte-macrophage colony stimulating factor | MGC131935 | MGC138897 | Mol

rp186551-10μg

10μg

无动物源

≥95%(SDS-PAGE)

GM‑CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte‑macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM‑CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM‑CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM‑CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy‑induced neutropenia, and GM‑CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM‑CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices. Mature human GM‑CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM‑CSF and 54% with mouse GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high‑affinity receptors for IL‑3 and IL‑5. In addition, GM‑CSF binds a naturally occurring soluble form of GM‑CSF R alpha. Human GM‑CSF is active on canine and feline cells but not on murine cells.

Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 90.7 pg/mL.

重组表达

14.5 kDa

No tag

16.5-28.2 kDa, under reducing conditions; 16.5-28.2 kDa, under non-reducing conditions.

HEK293 Accession #: P04141 | HEK293

<1.0 EU/μg

人(Human)

18-144 aa

GAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE​

Yes

Yes