Recombinant Human IL-18 Protein

重组人IL-18蛋白 | 重组人白介素-18蛋白

Iboctadekin | IFN-gamma-inducing factor | IGIF | IGIFIL-1 gamma | IL18 | IL-18 | IL-18MGC12320 | IL-1F4 | IL1F4iboctadekin | IL-1g | Interferon gamma-inducing factor | interleukin 18 (interferon-gamma-inducing factor) | Interleukin-1 gamma | interleukin-1

rp186564-10μg

10μg

无载体

≥95%(SDS-PAGE)

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. Post-translational: The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18.

Immobilized Recombinant Human IL-18 Bpa Protein (rp169678) at 2.0 μg/mL can bind Recombinant Human IL-18 Protein (rp186564) with the EC50 of 30.0 ng/mL.​ Immobilized Recombinant IL-18 protein (rp186564) at 1.0 μg/mL can bind Camoteskimab (anti-IL-18) (Ab182692) with the EC50 of 400.2 ng/mL.​

重组表达

18.2 kDa

No tag

15.7 kDa, under reducing conditions; 15.7 kDa, under non-reducing conditions.

E. coli Accession #: Q14116 | E. coli

<1.0 EU/μg

人(Human)

37-193 aa

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

No

Yes