Recombinant Human RIPK3 Protein

重组人RIPK3蛋白 | 重组人丝氨酸/苏氨酸蛋白激酶蛋白

Receptor interacting protein 3 | Receptor interacting serine threonine kinase 3 | Receptor interacting serine/threonine protein kinase 3 | Receptor-interacting protein 3 | Receptor-interacting serine/threonine-protein kinase 3 | RIP 3 | RIP like protein k

rp186577-10μg

10μg

无载体

≥95%(SDS-PAGE)

Necroptosis is a form of programmed necrotic cell death that is rapidly emerging as an important pathophysiological pathway in numerous disease states. Necroptosis is dependent on receptor-interacting protein kinase 3 (RIPK3), a protein shown to play an important role in experimental models of critical illness. RIPK3 promotes kidney tubular injury via mitochondrial dysfunction. The FAO-dependent RIPK3 mediates pathogenesis of acute lung injury. Post-translational: (Microbial infection) Proteolytically cleaved by S.flexneri OspD3 within the RIP homotypic interaction motif (RHIM), leading to its degradation and inhibition of necroptosis.RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (PubMed:19524513).Autophosphorylated following interaction with ZBP1 (By similarity). Phosphorylation of Ser-199 plays a role in the necroptotic function of RIPK3 (PubMed:11734559, PubMed:19524512).Autophosphorylates at Ser-227 following activation by ZBP1: phosphorylation at these sites is a hallmark of necroptosis and is required for binding MLKL (PubMed:22265413).Phosphorylation at Thr-182 is important for its kinase activity, interaction with PELI1 and PELI1-mediated 'Lys-48'-linked polyubiquitination and for its ability to mediate TNF-induced necroptosis (PubMed:29883609).Polyubiquitinated with 'Lys-48' and 'Lys-63'-linked chains by BIRC2/c-IAP1 and BIRC3/c-IAP2, leading to activation of NF-kappa-B (PubMed:21931591).Polyubiquitinated with 'Lys-48'-linked chains by PELI1 leading to its subsequent proteasome-dependent degradation. Ubiquitinated by STUB1 leading to its subsequent proteasome-dependent degradation (PubMed:29883609).Deubiquitinated by USP22 (PubMed:33369872).

重组表达

24.7 kDa

N-His

20.5 kDa, under reducing conditions

E. coli Accession #: Q9Y572 | E. coli

<1.0 EU/μg

人(Human)

2-218 aa

MHHHHHHSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGR

No

Yes