Recombinant Human TRAIL/TNFSF10 Protein

重组人TRAIL/TNFSF10蛋白 | 重组人TRAIL蛋白

TRAILTNF-related apoptosis inducing ligand TRAIL | tumor necrosis factor (ligand) superfamily, member 10 | tumor necrosis factor ligand superfamily member 10 | Apo-2 ligand | APO2L | Apo-2Ltumor necrosis factor (ligand) family, member 10 | APO2Ltumor necr

rp186865-10μg

10μg

无载体

≥95%(SDS-PAGE)

Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is the most homologous to Fas ligand, sharing 28% aa sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% aa sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that transduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified. Osteoprotegerin has been identified as a fifth TRAIL receptor. It was shown that homotrimeric TRAIL binds a zinc ion which is critical for the correct structure of the protein.

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 19.98 ng/mL.

重组表达

20.4 kDa

N-His

18.7 kDa, reducing conditions

E. coli Accession #: P50591 | E. coli

<1.0 EU/μg

人(Human)

114-281aa

MHHHHHHVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG

No

Yes