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Product details

名称

Recombinant Human CDK2 Protein

别名

重组人CDK2蛋白 | 重组人细胞周期蛋白依赖性激酶2

英文别名

货号

rp188224-10μg

包装规格

10μg

级别

无载体

浓度

≥95%(SDS-PAGE)

生化机理

Cyclin-dependent kinase 2 (CDK2) belongs the Ser/Thr protein kinase family. CDK2 is highly parallel to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is limited to the G1-S phase, and is vital for cell cycle G1/S phase transition. The CDK2 protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 activity is also regulated by protein phosphorylation. Post-translational: Phosphorylated at Thr-160 by CDK7 in a CAK complex (PubMed:28666995). Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A. Nitrosylated after treatment with nitric oxide (DETA-NO).

生物活性

Immobilized Recombinant Human CDK2 Protein (rp188224) at 1.0 μg/mL can bind CDK2 Mouse mAb (Ab095749) with the EC50 of 62.4 ng/mL.

来源

重组表达

预测分子量

34.8 kDa

蛋白标签

N-His

SDS-PAGE

33.2 kDa, under reducing conditions; 33.2 kDa, under non-reducing conditions.

表达系统

E. coli Accession #: P24941 | E. coli

内毒素水平

<1.0 EU/μg

种属

人(Human)

氨基酸

2-298 aa

序列

MHHHHHHENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

无动物源

无载体

Yes

Safety

「No Dangerous Attributes」

「No upstream and downstream information yet」

Technical Documents

「No technical documents」

Articles

「No related articles yet」

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