Recombinant Human GABARAP Protein

重组人GABARAP蛋白 | 重组人γ-氨基丁酸受体相关蛋白

ATG8A Protein, Human | FLC3B Protein, Human | GABARAP-a Protein, Human | HT004 Protein, Human | MM46 Protein, Human

rp188254-10μg

10μg

无载体

≥95%(SDS-PAGE)

The Atg8/LC3/GABARAP family of proteins, a group that has structural homology with ubiquitin, connects with a large set of binding partners to function in macroautophagy (hereafter autophagy). GABARAP in tumorigenesis in vivo by delaying cell death and its associated immune-related response. GABARAPs are uniquely required for antimicrobial host defense through cytosolic distribution of interferon-inducible GTPases. GABARAPs as the first known direct interaction partners of Nef that are essential for its plasma membrane localization. Post-translational: The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAP-I (PubMed:15169837, PubMed:20818167, PubMed:30661429). The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAP-II (PubMed:15169837). During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989). ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:33909989). In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989). ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429). ATG4D is the main enzyme for delipidation activity (By similarity). (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:31722778). RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989).

Immobilized Recombinant Human GABARAP Protein (rp188254) at 1.0 μg/mL can bind Recombinant GABARAP Antibody (Ab104437) with the EC50 of 7.0 ng/mL.

重组表达

14.7 kDa

N-His

14.6 kDa, under reducing conditions; 14.6 kDa, under non-reducing conditions.

E. coli Accession #: O95166 | E. coli

<1.0 EU/μg

人(Human)

2-117 aa

MHHHHHHKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL

No

Yes