Recombinant Human Brd4 Protein

重组人Brd4蛋白 | 重组人溴结构域4蛋白

BRD4 | bromodomain containing 4 | bromodomain-containing 4 | CAPchromosome-associated protein | HUNK1 | HUNK1bromodomain-containing protein 4 | HUNKI | MCAP | Protein HUNK1

rp188282-10μg

10μg

无载体

≥95%(SDS-PAGE)

Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others. This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains. This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders. Post-translational: Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones. In absence of phosphorylation, BRD4 does not localize to p53/TP53 target gene promoters, phosphorylation promoting recruitment to p53/TP53 target promoters.

Immobilized Recombinant Human Brd4 Protein (rp188282) at 1.0 μg/mL can bind Recombinant Brd4 Antibody (Ab091675) with the EC50 of 8.4 ng/mL.

重组表达

48.0 kDa

N-10His

52.8 kDa, under reducing conditions; 52.8 kDa, under non-reducing conditions.

E. coli Accession #: O60885 | E. coli

<1.0 EU/μg

人(Human)

49-460 aa

MHHHHHHHHHHETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTPPQTQTPQPNPPPVQATPHPFPAVTPDLIVQTPVMTVVPPQPLQTPPPVPPQPQPPPAPAPQPVQSHPPIIAATPQPVKTKKGVKRKADTTTPTTIDPIHEPPSLPPEPKTTKLGQRRESSRPVKPPKKDVPDSQQHPAPEKSSKVSEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLEAREYRDAQEFGADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDE

No

Yes