Recombinant Human dUTPase Protein

重组人dUTPase蛋白

Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial | Deoxyuridine triphosphatase | Dut | DUT_HUMAN | dUTP nucleotidohydrolase | dUTP pyrophosphatase | dUTPase | FLJ20622

rp188377-10μg

10μg

无载体

≥95%(SDS-PAGE)

Deoxyuridine Triphosphatase (DUT) is a ubiquitous enzyme that functions in nucleotide metabolism. Deoxyuridine Triphosphatase, in the presence of magnesium ions, is responsible for hydrolyzing dUTP to dUMP and diphosphate. This reaction is imperative for keeping the intracellular dUTP concentration low so that uracil does not become incorporated into DNA. Extensive integration of uracil into DNA can eventually lead to cell death. This suggests that DUT is crucial for cell viability, further implying that it is a prospective target for anticancer therapy. Post-translational: Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.

重组表达

18.2 kDa

N-His

18.2 kDa, under reducing conditions; 17.7 & 38.4 kDa, under non-reducing conditions.

E. coli Accession #: P33316 | E. coli

<1.0 EU/μg

人(Human)

94-252 aa

MHHHHHHETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN​

No

Yes