Recombinant Human GRB2 Protein

重组人GRB2蛋白 | 重组人生长因子受体结合蛋白2

Abundant SRC homology | Adapter protein GRB2 | ASH | Ash protein | EGFRBP GRB2 | Epidermal growth factor receptor binding protein | Epidermal growth factor receptor binding protein GRB2 | GRB 2 | Grb2 | GRB2 adapter protein | GRB2_HUMAN | Grb3 3 | Growth

rp188402-10μg

10μg

无载体

≥90%(SDS-PAGE)

GRB2 is widely expressed and binds the EGFR and contains one SH2 domain and two SH3 domains which form a complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. GRB2 is related to the Sem5 gene of C.elegans, which is takes part in the signal transduction pathway. GRB2 is an adaptor protein that provides an important link between cell surface growth factor receptors and the Ras signaling pathway. Inhibition of GRB2 activity damages developmental processes in a variety of organisms and blocks transformation and proliferation of different cell types. Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. Post-translational: Phosphorylation of Tyr-209 in the C-terminal SH3 domain reduces its binding to SOS1. Ubiquitinated by RNF173, leading to proteasomal degradation and inhibition of the RAF/MEK/ERK pathway (PubMed:37328606). In the nucleus, polyubiquitinated by RBBP6 at Lys-109 at DNA damage sites (PubMed:34348893).

Immobilized Recombinant Human GRB2 Protein (rp188402) at 1.0 μg/mL can bind GRB2 Mouse mAb (Ab106387) with the EC50 of 30.5 ng/mL.

重组表达

26.0 kDa

N-His

26.3 kDa, under reducing conditions; 24.6 & 26.7 & 49.3 kDa, under non-reducing conditions.

E. coli Accession #: P62993 | E. coli

<1.0 EU/μg

人(Human)

2-217 aa

MHHHHHHEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

No

Yes