Recombinant Human Gankyrin Protein

重组人Gankyrin蛋白 | 重组人26S蛋白酶体非ATP酶调节亚基10蛋白

26S proteasome non-ATPase regulatory subunit 10 | 26S proteasome regulatory subunit p28 | Ankyrin repeat protein | dJ889N15.2 | Gankyrin | Hepatocellular carcinoma-associated protein p28 II | p28 | p28(GANK) | Proteasome (prosome, macropain) 26S subunit,

rp188418-10μg

10μg

无载体

≥90%(SDS-PAGE)

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.

Immobilized Recombinant Human Gankyrin Protein (rp188418) at 1.0 μg/mL can bind Gankyrin Mouse mAb (Ab104742) with the EC50 of 59.5 ng/mL.

重组表达

24.3 kDa

No tag

24.3 kDa, under reducing conditions; 24.9 kDa, under non-reducing conditions

E. coli Accession #: O75832 | E. coli

<1.0 EU/μg

人(Human)

2-226 aa

EGCVSNLMVCNLAYSGKLEELKESILADKSLATRTDQDSRTALHWACSAGHTEIVEFLLQLGVPVNDKDDAGWSPLHIAASAGRDEIVKALLGKGAQVNAVNQNGCTPLHYAASKNRHEIAVMLLEGGANPDAKDHYEATAMHRAAAKGNLKMIHILLYYKASTNIQDTEGNTPLHLACDEERVEEAKLLVSQGASIYIENKEEKTPLQVAKGGLGLILKRMVEG

No

Yes