Recombinant Human ENPP-6 Protein

重组人ENPP-6蛋白 | 重组人外核苷酸焦磷酸酶/磷酸二酯酶6蛋白

B830047L21Rik | EC 3.1 | ectonucleotide pyrophosphatase/phosphodiesterase 6 | ectonucleotide pyrophosphatase/phosphodiesterase family member 6 | E-NPP 6 | ENPP6 | ENPP-6 | GPC-Cpde | MGC33971 | NPP6 | NPP-6

rp188461-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Ectonucleotide Pyrophosphatase/Phosphodiesterase 6 (ENPP-6) is a choline-specific glycerophosphodiester phosphodiesterase. It is a member of a family of membrane-linked glycoproteins that, at an alkaline pH, hydrolyze pyrophosphate or phosphodiester bonds in a variety of extracellular compounds including nucleotides, lysophospholipids, and choline phosphate esters. ENPP-6 is a GPI-linked protein that is synthesized as a 440 amino acid (aa) precursor and has a predicted molecular weight of approximately 55 kDa. Its extracellular region contains a catalytic domain that is nearly 400 aa in length and shares 88% aa sequence identity with the mouse and rat orthologs. A soluble form of ENPP-6 can be proteolytically shed and associate into a disulfide-linked homodimer.The catalytic domain of ENPP-6 specifically recognizes the phosphocholine part of its substrate. ENPP-6 has been shown to display preference for choline-containing phospholipids or phosphodiesters such as lysophosphatidylcholine (LPC), glycerophosphorylcholine (GPC), sphingosylphosphorylcholine (SPC), Platelet-Activating Factor (PAF), and lysoPAF. Furthermore, ENPP-6 shows preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. In vitro, ENNP-6 has been shown to efficiently hydrolyze the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, but not the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. ENPP-6 is predominantly expressed in brain, where it is localized to myelin and kidney, with lesser expression being found in the heart. ENPP-6 expression in the brain has been shown to be regulated by Thyroid Hormone and iron. Additionally, in rat brain, ENPP-6 expression has been shown to be up-regulated during oligodendrocyte differentiation.

Measured by its ability to cleave O-(4-Nitrophenylphosphoryl) choline. The specific activity is >4,000 pmol/min/μg, as measured under the described conditions.

重组表达

47.3 kDa

C-10His

52.2 kDa, under reducing conditions; 51.6 & 105.0 kDa, under non-reducing conditions.

HEK293 Accession #: Q6UWR7 | HEK293

<1.0 EU/μg

人(Human)

1-419 aa

MAVKLGTLLLALALGLAQPASARRKLLVFLLDGFRSDYISDEALESLPGFKEIVSRGVKVDYLTPDFPSLSYPNYYTLMTGRHCEVHQMIGNYMWDPTTNKSFDIGVNKDSLMPLWWNGSEPLWVTLTKAKRKVYMYYWPGCEVEILGVRPTYCLEYKNVPTDINFANAVSDALDSFKSGRADLAAIYHERIDVEGHHYGPASPQRKDALKAVDTVLKYMTKWIQERGLQDRLNVIIFSDHGMTDIFWMDKVIELNKYISLNDLQQVKDRGPVVSLWPAPGKHSEIYNKLSTVEHMTVYEKEAIPSRFYYKKGKFVSPLTLVADEGWFITENREMLPFWMNSTGRREGWQRGWHGYDNELMDMRGIFLAFGPDFKSNFRAAPIRSVDVYNVMCNVVGITPLPNNGSWSRVMCMLKGRASHHHH​HHHHHH

Yes

Yes