Recombinant Human C2GNT3/GCNT4 Protein

重组人C2GNT3/GCNT4蛋白 | 重组人葡糖胺基 (N-乙酰基) 转移酶4蛋白

beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 4 | C2GNT3 | core 2 beta-1,6-N-acetylglucosaminyltransferase 3 | Core 2-branching enzyme 3 | Core2-GlcNAc-transferase 3 | EC 2.4.1.102 | GCNT4 | glucosaminyl (N-acetyl) tr

rp188463-10μg

10μg

无动物源

≥95%(SDS-PAGE)

Mucin-type O-glycans are initiated with an O-GalNAc attachment to a serine or threonine in a polypeptide. The O-GalNAc residues are subsequently extended by various glycosyltransferases resulting in different types of O-glycans. Most O-glycans contain the core 1 structure, Gal beta 1-3GalNAc. GCNT4 is a member of the glucosaminyl (N-acetyl) transferase family and it converts the core 1 O-glycan to core 2 O-glycan, Gal beta 1-3(GlcNAc beta 1-6) GalNAc, via the addition of a GlcNAc residue. Various ligand carbohydrates can be formed from core 2 branched oligosaccharides. For example, sialyl Lex in mucin‑type glycoproteins of blood cells can be formed from core 2 branched oligosaccharides. GCNT4 does not have core 4 O-glycan or I-branching enzyme activity. GCNT4 has been shown to play a role in gastric cancers, where GCNT4 is downregulated and its overexpression prevents cancer cell growth. The activity of the recombinant human GCNT-4 was determined using a phosphatase‑coupled glycosyltransferase assay.

Testing in progress

重组表达

49.7 kDa

C-His

59.9 kDa, under reducing conditions; 62.1 & 122.2 kDa, under non-reducing conditions.

CHO Accession #: Q9P109 | CHO

<1.0 EU/μg

人(Human)

35-453 aa

RRLFPQKDIYLVEYSLSTSPFVRNRYTHVKDEVRYEVNCSGIYEQEPLEIGKSLEIRRRDIIDLEDDDVVAMTSDCDIYQTLRGYAQKLVSKEEKSFPIAYSLVVHKDAIMVERLIHAIYNQHNIYCIHYDRKAPDTFKVAMNNLAKCFSNIFIASKLEAVEYAHISRLQADLNCLSDLLKSSIQWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPNSKLERFTYHHELRRVPYEYVKLPIRTNISKEAPPHNIQIFVGSAYFVLSQAFVKYIFNNSIVQDFFAWSKDTYSPDEHFWATLIRVPGIPGEISRSAQDVSDLQSKTRLVKWNYYEGFFYPSCTGSHLRSVCIYGAAELRWLIKDGHWFANKFDSKVDPILIKCLAEKLEEQQRDWITLPSEKLFMDRNLTTTSHHHHHH

Yes

Yes