Recombinant Human UAP56 Protein

重组人UAP56蛋白 | 重组人剪接体RNA解旋酶DDX39B蛋白

0610030D10Rik | 4F2-LC6 | 56 kDa U2AF65-associated protein | AI428441 | ATP-dependent RNA helicase p47 | B(0,+)-type amino acid transporter 1 | BAT1 | Bat1a | D17H6S81E | D17H6S81E-1 | D6S81E | D6S81Eh | DDX39B | DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B

rp188494-10μg

10μg

无载体

≥90%(SDS-PAGE)

Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

Immobilized Recombinant Human UAP56 Protein (rp188494) at 1.0 μg/mL can bind UAP56 Antibodydy (Ab133187) with the EC50 of 48.8 ng/mL.

重组表达

49.8 kDa

N-His

49.1 kDa, under reducing conditions; 49.1 kDa, under non-reducing conditions.

E. coli Accession #: Q13838 | E. coli

<1.0 EU/μg

人(Human)

2-428 aa

MHHHHHHAENDVDNELLDYEDDEVETAAGGDGAEAPAKKDVKGSYVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAILGMDVLCQAKSGMGKTAVFVLATLQQLEPVTGQVSVLVMCHTRELAFQISKEYERFSKYMPNVKVAVFFGGLSIKKDEEVLKKNCPHIVVGTPGRILALARNKSLNLKHIKHFILDECDKMLEQLDMRRDVQEIFRMTPHEKQVMMFSATLSKEIRPVCRKFMQDPMEIFVDDETKLTLHGLQQYYVKLKDNEKNRKLFDLLDVLEFNQVVIFVKSVQRCIALAQLLVEQNFPAIAIHRGMPQEERLSRYQQFKDFQRRILVATNLFGRGMDIERVNIAFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNDVQDRFEVNISELPDEIDISSYIEQTR

No

Yes