Recombinant Human CD40 Ligand/TNFSF5 Protein

重组人CD40 Ligand/TNFSF5蛋白 | 重组人 CD40 配体/TNFSF5 蛋白

CD154 | CD40 antigen ligand | CD40 Ligand | CD40L | CD40-L | CD40LG | CD40LIGM | gp39 | CD154 antigen | hCD40L | HIGM1 | T-B cell-activating molecule | T-BAM | T-cell antigen Gp39 | TNF-related activation protein | TNFSF5 | TNFSF5IMD3 | TRAP | TRAPtumor n

rp189423-10μg

10μg

无载体

≥95%(SDS-PAGE)

Protein Function: CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp169583) with the EC50 of 25.02 ng/mL.

重组表达

17.7 kDa

N-His

15.4 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions

E. coli Accession #: P29965 | E. coli

<1.0 EU/μg

人(Human)

108-261 aa

MHHHHHHENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

No

Yes