Recombinant Human B7-1/CD80 Protein

重组人B7-1/CD80蛋白 | 重组人T淋巴细胞激活抗原CD80蛋白

Activation B7-1 antigen | B7 | B71 | B7-1 | BB1 | CD28LG1 | CD28LGB7-1 antigen) | CD80 antigen | CD80 molecule | CD80 | costimulatory factor CD80 | costimulatory molecule variant IgV-CD80 | CTLA-4 counter-receptor B7.1 | T-lymphocyte activation antigen CD

rp189426-10μg

10μg

无动物源

≥90%(SDS-PAGE)

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature human B7-1 consists of a 208 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Within the ECD, human B7-1 shares 50% aa sequence identity with mouse B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Post-translational: Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization.

Immobilized Recombinant Human CD80 Protein (rp189426) at 1.0 μg/mL can bind Galiximab (anti-CD80) (Ab177850) with the EC50 of 95.6 ng/mL.

重组表达

24.6 kDa

C-His

39.2-48.6 kDa, under reducing conditions; 38.5-47.5 kDa, under non-reducing conditions.

HEK293 Accession #: P33681 | HEK293

<1.0 EU/μg

人(Human)

1-242 aa

MGHTRRQGTSPSKCPYLNFFQLLVLAGLSHFCSGVIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNHHHHHH

Yes

Yes